Simple and Sensitive Lc–uv Method for Simultaneous Analysis of Hydrochlorothiazide and Candesartan Cilexetil in Pharmaceutical Formulations
نویسندگان
چکیده
A simple, sensitive, and inexpensive high-performance liquid-chromatographic method has been developed for simultaneous determination of hydrochlorothiazide and candesartan cilexetil in pharmaceutical formulations. Chromatographic separation was achieved on a Phenyl-2 column with a 25:75:0.2 mixture of 0.02 M potassium dihydrogen phosphate, methanol, and triethylamine, final pH 6.0 ± 0.1, as mobile phase. Detection was at 271 nm. Response was a linear function of concentration in the range 5–45 μg mL for hydrochlorothiazide and 12–56 μg mL for candesartan cilexetil; the correlation coefficients were 0.9993 and 0.9991, respectively. Total elution time for the two components was less than 5 min.
منابع مشابه
DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR ESTIMATION OF HYDROCHLOROTHIAZIDE AND CANDESARTAN CILEXETIL IN PHARMACEUTICAL DOSAGE FORM *Ananda Rao Bonthala and T.A.D
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